POSTDOC_Species-Specific ADAT Function in Cortical Development_061_JG - H/F

Description entreprise :

L’Inserm est le seul organisme public français entièrement dédié à la recherche biologique, médicale et en santé des populations. Il dispose de laboratoires de recherche sur l’ensemble du territoire, regroupés en 12 Délégations Régionales. Notre institut réunit 15 000 chercheurs, ingénieurs, techniciens et personnels administratifs, avec un objectif commun : améliorer la santé de tous par le progrès des connaissances sur le vivant et sur les maladies, l’innovation dans les traitements et la recherche en santé publique.

Rejoindre l’Inserm, c’est intégrer un institut engagé pour la parité et l’égalité professionnelle, la diversité et l’accompagnement de ses agents en situation de handicap, dès le recrutement et tout au long de la carrière. Afin de préserver le bien-être au travail, l’Inserm mène une politique active en matière de conditions de travail, reposant notamment sur un juste équilibre entre vie personnelle et vie professionnelle.

L'Inserm a reçu en 2016 le label européen HR Excellence in Research et s'est engagé à faire évoluer ses pratiques de recrutement et d'évaluation des chercheurs.

Description du poste :

We are looking for a highly motivated postdoctoral researcher to join the “Physiological and Pathological Mechanisms of Cortical Development” team led by Dr. Juliette Godin at the Institute of Genetics and Molecular and Cellular Biology (IGBMC) in Strasbourg, France. The Godin lab is a recognized expert in translational control during cortical development. The selected candidate will work under the direct supervision of Dr. Efil Bayam (CRCN Inserm), in the framework of the ANR-funded project SpeaDAT, which investigates the species-specific mechanisms of ADAT tRNA deaminase complex dysfunction in brain development. The postdoctoral researcher will study species-specific ADAT functions in progenitors and neurons and characterize impact of ADAT dysfunction on tRNAs and translational programs using human iPSC- and mouse ESCs -derived models, including 2D cortical neural stem cells/neurons and 3D cortical organoids carrying ADAT2 and ADAT3 pathogenic variants. This 30-month position is well suited to a recently graduated PhD experienced with human iPSC culture and/or 2D/3D neural differentiation protocols.

The candidate will generate and characterize human iPSC-derived 2D cortical neural stem cells, neurons and 3D cortical organoids carrying ADAT2 and ADAT3 pathogenic variants, assess cellular phenotypes (proliferation, cell cycle dynamics, progenitor identity, neuronal maturation and synaptic integration) using immunofluorescence, EdU/pulse-chase labeling and functional assays (MEA, calcium imaging) and analyze and interpret human mim-tRNA-seq and ribosome profiling data to characterize species-specific tRNA and translational programs in close collaboration with a bioinformatician.

Present research findings at team and consortium meetings and at national/international conferences, contribute to manuscript writing and to project reporting; contribute to the supervision of students.

Profil recherché :

Skills/Qualifications :

• Ph.D in Cell Biology, Molecular Biology, Developmental Biology, Neuroscience or a related field, recently obtained.
• Experience with human iPSC culture and/or 2D/3D neural differentiation protocols is required.
• Proficiency in standard molecular and cell biology techniques (immunofluorescence, western blot, qPCR, imaging)
• Familiarity with high-throughput sequencing approaches (RNA-seq, ribosome profiling) is a plus
• Interest in neurodevelopment, neurodevelopmental disorders and translational control
• Fast learner, critical thinking, positive attitude, strong problem-solving skills and adaptability to new data and questions
• Good interpersonal skills and ability to work within an interdisciplinary, international consortium
• Strong written and oral communication skills in English
• Strong organizational skills, personal motivation and creativity

English (fluent, required).

Required Experience : 0 - 2 years, fields: Cell biology, molecular biology, developmental biology, neuroscience, stem cell biology or a related field.